Journal Article

Tandem arrayed Iigation of expressed sequence tags (TALEST): a new method for generating global gene expression profiles

Dominic G. Spinella, Alexandra K. Bernardino, Amy C. Redding, Patricia Koutz, Yalin Wei, Ellen K. Pratt, Kristi K. Myers, Gary Chappell, Steven Gerken and Stephen J. McConnell

in Nucleic Acids Research

Volume 27, issue 18, pages e22-i-e22-viii
Published in print September 1999 | ISSN: 0305-1048
Published online September 1999 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/27.18.e22-i
Tandem arrayed Iigation of expressed sequence tags (TALEST): a new method for generating global gene expression profiles

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We have developed a new and simple method for quantitatively analyzing global gene expression profiles from cells or tissues. The process, called TALEST, or tandem arrayed ligation of expressed sequence tags, employs an oligonucleotide adapter containing a type IIs restriction enzyme site to facilitate the generation of short (16 bp) ESTs of fixed position in the mRNA. These ESTs are flanked by GC-clamped punctuation sequences which render them resistant to thermal denaturation, allowing their concatenation into long arrays and subsequent recognition and analysis by high-throughput DNA sequencing. A major advantage of the TALEST technique is the avoidance of PCR in all stages of the process and hence the attendant sequence-specific amplification biases that are inherent in other gene expression profiling methods such as SAGE, Differential Display, AFLP, etc. which rely on PCR.

Journal Article.  5080 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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