Journal Article

Synthesis and processing of tRNA-related SINE transcripts in Arabidopsis thaliana

Thierry Pélissier, Cécile Bousquet-Antonelli, Laurence Lavie and Jean-Marc Deragon

in Nucleic Acids Research

Volume 32, issue 13, pages 3957-3966
Published in print January 2004 | ISSN: 0305-1048
Published online January 2004 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/gkh738
Synthesis and processing of tRNA-related SINE transcripts in Arabidopsis thaliana

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Despite the ubiquitous distribution of tRNA-related short interspersed elements (SINEs) in eukaryotic species, very little is known about the synthesis and processing of their RNAs. In this work, we have characterized in detail the different RNA populations resulting from the expression of a tRNA-related SINE S1 founder copy in Arabidopsis thaliana. The main population is composed of poly(A)-ending (pa) SINE RNAs, while two minor populations correspond to full-length (fl) or poly(A) minus [small cytoplasmic (sc)] SINE RNAs. Part of the poly(A) minus RNAs is modified by 3′-terminal addition of C or CA nucleotides. All three RNA populations accumulate in the cytoplasm. Using a mutagenesis approach, we show that the poly(A) region and the 3′ end unique region, present at the founder locus, are both important for the maturation and the steady-state accumulation of the different S1 RNA populations. The observation that primary SINE transcripts can be post-transcriptionally processed in vivo into a poly(A)-ending species introduces the possibility that this paRNA is used as a retroposition intermediate.

Journal Article.  8394 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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