Journal Article

Characterization of pro-apoptotic gene Bak using hammerhead ribozymes

Eigo Suyama, Hiroaki Kawasaki and Kazunari Taira

in Nucleic Acids Symposium Series

Volume 1, issue 1, pages 207-208
Published in print November 2001 | ISSN: 0261-3166
Published online November 2001 | e-ISSN: 1746-8272 | DOI: https://dx.doi.org/10.1093/nass/1.1.207
Characterization of pro-apoptotic gene Bak using hammerhead ribozymes

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  • Chemistry
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Bak gene encodes a 23kDa-protein homologue of apoptosis inhibitor Bcl-2. Unlike Bcl-2, Bak stimulates several apoptosis pathways, and by interacting with Bcl-2 or Bcl-xL, it counteracts the anti-cell death effect. Enforced expression of Bak induces rapid and extensive apoptosis of serum-deprived fibroblasts. In contrast, the mutation of the Bak gene was observed in several cancer cells, suggesting a deficiency of the Bak cellular function can cause a cancerous malignancy. Although many studies of Bak have been performed, the mechanisms by which Bak functions in mammalian cells remain unclear. In this study, we constructed a hammerhead ribozyme specifically targeted to the Bak mRNA for inhibition of the intracellular function of Bak. This Bak-ribozyme should be useful for a detailed functional analysis of Bak.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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