In vitro selection has been used as a method to determine the optimal binding site for DNA-inding proteins. We report here in vitro selection of dsDNA sequences that bind to mutated-GCN4-bZIP peptides. The GCN4-bZIP peptide mutated from alanine to histidine on a position −14 that contacts with DNA bound to different sequence from a binding site of wild type peptide.
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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology
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