Journal Article

Purification and Characterization of a Novel Isoform of Mast Cell Tryptase from Rat Tongue

Yuushi Okumura, Asako Kudoh, Miwa Takashima, Masahiro Inoue, Kentaro Sakai and Hiroshi Kido

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 120, issue 4, pages 856-864
Published in print October 1996 | ISSN: 0021-924X
Published online October 1996 | e-ISSN: 1756-2651 | DOI: https://dx.doi.org/10.1093/oxfordjournals.jbchem.a021491
Purification and Characterization of a Novel Isoform of Mast Cell Tryptase from Rat Tongue

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Rat mast cell tryptase was purified to homogeneity from rat tongue by a series of standard chromatographic procedures. Since the enzyme gave band corresponding to molecular mass of 32–35 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis and exhibited a molecular mass of 135 kDa on gel filtration, it was presumed to be a noncovalently associated tetramer. The N-terminal amino acid sequence of 50 residues of the enzyme showed the highest degree of homology with the same region in mouse mast cell protease 7 (92%), and less homology to those of tryptases from man and dog, and peritoneal cells of rats and Mongolian gerbils. The inhibitor specificity of rat tongue tryptase was similar to that of rat peritoneal mast cell tryptase free from trypstatin: it was inhibited by α1-antitrypsin, Kunitz-type soybean trypsin inhibitor and Bowman-Birk soybean trypsin inhibitor, but these inhibitors do not inhibit the tryptases from rat skin, human lung, and dog mast cells. Judging from these results, together with other enzymatic properties, the enzyme may be a novel isoform of tryptase in rat tongue. Analysis by differential staining with peroxidaselabeled lectins of the enzyme suggested that it has tri- and/or tetraantennary complex-type oligosaccharides containing a relatively high amount of sialic acid. The immunohistochemical distribution of this enzyme indicated that the reactive antigen was specific in connective tissue but not in mucosal mast cells.

Keywords: glycosylation; mast cell; N-terminal amino acid sequence; rat tongue; tryptase

Journal Article.  0 words. 

Subjects: Biochemistry

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