Journal Article

Cationic Liposome-Mediated Efficient Induction of Type I Interferons by a Low Dose of Poly I: Poly C in Mouse Cell Lines

Sachiko Okamoto, Yoshihiko Watanabe, Yoshinobu Takakura and Mitsuru Hashida

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 124, issue 4, pages 697-701
Published in print October 1998 | ISSN: 0021-924X
Published online October 1998 | e-ISSN: 1756-2651 | DOI: https://dx.doi.org/10.1093/oxfordjournals.jbchem.a022168

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Double-stranded polyriboinosinic acid:polyribocytidylic acid (poly I:poly C) is a powerful inducer of type I interferons (IFNs). However, the dose of poly I:poly C required for efficient IFN induction is so high as occasionally to be cytotoxic. In this work, we examined the IFN-inducibility of poly I:poly C complexed with several cationic reagents in mouse fibroblast L cells and found that Lipofectin and LipofectACE can induce the production of a substantial amount of type I IFNs (mostly β-type) even at a two-order lower dose compared with poly I:poly C alone. Such effects of poly I:poly C were optimal at 0.1 μg/ml for 2–10 μg/ml of Lipofectin and LipofectACE. These conditions caused no significant cytotoxicity in the recipient cells. Furthermore, a short treatment (less than 10 min) with the complexes was sufficient for the maximum induction. This IFN induction method was applicable to other cell types and other species including human. Hence, our observations may pave the way for clinical application of the IFN inducer.

Keywords: cationic liposomes; efficient IFN induction; FITC-poly I:poly C; interferon; poly I:poly C

Journal Article.  0 words. 

Subjects: Biochemistry