Journal Article

Genetic interactions between protein phosphatases CaPtc2p and CaPph3p in response to genotoxins and rapamycin in Candida albicans

Jinrong Feng, Yunying Zhao, Yinong Duan and Linghuo Jiang

in FEMS Yeast Research

Volume 13, issue 1, pages 85-96
Published in print February 2013 |
Published online May 2013 | e-ISSN: 1567-1364 | DOI: https://dx.doi.org/10.1111/1567-1364.12012

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In Saccharomyces cerevisiae cells, both of the two PP2C protein phosphatases ScPtc2p and ScPtc3p and the PP4 protein phosphatase ScPph3 are responsible for ScRad53p dephosphorylation after the DNA methylation agent methylmethane sulfonate (MMS)-induced DNA damage. In this study, we show that CaPtc2p is not required for the CaRad53p dephosphorylation during the recovery from DNA damage, as is CaPph3p in Candida albicans. However, deletion of CaPPH3 has an additive effect on the sensitivity of C. albicans cells lacking CaPTC2 to MMS and the DNA synthesis inhibitor hydroxyurea (HU). In addition, deletion of CaPPH3 promotes in vitro filamentation of C. albicans cells. Furthermore, mutation of CaPTC2 is epistatic to that of CaPPH3 in the sensitivity of C. albicans cells to rapamycin. Therefore, CaPtc2p and CaPph3p might play a role in the target of rapamycin (TOR) signaling in C. albicans cells.

Keywords: genotoxin; protein phosphatase; PTC2; PPH3; RAD53; rapamycin

Journal Article.  6636 words.  Illustrated.

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