Journal Article

The Escherichia coli modE gene: effect of modE mutations on molybdate dependent modA expression

Paul M. McNicholas, Robin C. Chiang and Robert P. Gunsalus

in FEMS Microbiology Letters

Volume 145, issue 1, pages 117-123
Published in print November 1996 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI:

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The Escherichia coli modABCD operon, which encodes a high-affinity molybdate uptake system, is transcriptionally regulated in response to molybdate availability by ModE. Here we describe a highly effective enrichment protocol, applicable to any gene with a repressor role, and establish its application in the isolation of transposon mutations in modE. In addition we show that disruption of the ModE C-terminus abolishes derepression in the absence of molybdate, implying this region of ModE controls the repressor activity. Finally, a mutational analysis of a proposed molybdate binding motif indicates that this motif does not function in regulating the repressor activity of ModE.

Keywords: modABCD operon; Transcriptional repressor; Molybdate binding domain

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