Journal Article

Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera)

V. Amore, M. A. Puig García, A. M. Timperio, G. Egidi, N. Ubero-Pascal and R. Fochetti

in Environmental Entomology

Published on behalf of Entomological Society of America

Volume 40, issue 1, pages 167-171
Published in print February 2011 | ISSN: 0046-225X
Published online November 2014 | e-ISSN: 1938-2936 | DOI: https://dx.doi.org/10.1603/EN10088

Show Summary Details

Preview

Hemocyanins are large oligomeric respiratory proteins found in many arthropods and mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer, 1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes (Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry. We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the hemocyanin is actually expressed in species in which its mRNA is present.

Keywords: copper binding proteins; nano-RP-HPLC-ESI-MS; insects

Journal Article.  3234 words.  Illustrated.

Subjects: Entomology